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human cot-1 dna tm  (Thermo Fisher)


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    Structured Review

    Thermo Fisher human cot-1 dna tm
    Reagents and tools table
    Human Cot 1 Dna Tm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cot+1+dna/human+cot+1+dna/pmc11628560-3-0-5
    Average 90 stars, based on 1 article reviews
    human cot-1 dna tm - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Aberrant fragmentomic features of circulating cell-free mitochondrial DNA as novel biomarkers for multi-cancer detection"

    Article Title: Aberrant fragmentomic features of circulating cell-free mitochondrial DNA as novel biomarkers for multi-cancer detection

    Journal: EMBO Molecular Medicine

    doi: 10.1038/s44321-024-00163-6

    Reagents and tools table
    Figure Legend Snippet: Reagents and tools table

    Techniques Used: Sequencing, Software

    Related Articles

    Hybridization:

    Article Title: Deconwolf enables high-performance deconvolution of widefield fluorescence microscopy images
    Article Snippet: We stored the cells in 2× SSC supplemented with 0.05% NaN 3 (Merck, cat. no. S2002) at +4 °C for up to 1 month until hybridization. .. Prior to hybridization, we incubated the cells in 50% formamide, 2× SSC and 50 mM phosphate buffer overnight at room temperature, followed by 1 h of incubation with a pre-hybridization buffer containing 50% formamide, 2× SSC, 5× Denhardt’s solution (Thermo Fisher Scientific, cat. no. 750018), 50 mM sodium phosphate buffer (home made from sodium dihydrogen phosphate (Merck, cat. no. 7558-80-7) and disodium hydrogen phosphate (Merck, cat. no. 7558-79-4)), 1 mM EDTA (Thermo Fisher Scientific, cat. no. AM9260G), 100 mg ml −1 salmon sperm DNA (Thermo Fisher Scientific, cat. no. 15632011) and 100 mg ml −1 human Cot-1 DNA (Thermo Fisher Scientific, cat. no. 15279011), pH 7.5–8 at 37 °C in a humidity chamber. ..

    Article Title: Methods for barcoding macromolecules in individual cells
    Article Snippet: Following washing, cells are incubated in pre-hybridization buffer, consisting of 2× saline-sodium citrate buffer (SSC; Ambion, AM9763), 50% formamide (Ambion, AM9342) and 0.1% of Tween-20 (Sigma-Aldrich, P4916) for 30 minutes. .. Next, the cells are incubated in hybridization buffer (2×SSC, 50% formamide, 10% dextran sulfate (Sigma-Aldrich, D8906) containing a mixture of CBPs at ̃20 nM total concentration with or without 10 μg Human Cot-1 DNA (ThermoFisher, 15279011). ..

    Article Title: Nucleic acid capture method
    Article Snippet: .. A hybridization mixture was prepared containing 500 ng DNA library, 2 ug of RNA probes (1 ug from “+” clone transcripts and 1 ug from “−” clone transcripts, targeting Hg19 human exomes including −50 bp and +50 bp flanking intronic sequences, as well as 5′ and 3′ UTRs as described before), 7 ul Human Cot-1 DNA (ThermoFisher Scientific), 3 ul Herring Sperm DNA Solution (ThermoFisher Scientific), 10 μl blocking Oligos (1 nmol/ul each) with following sequences: Blocking Oligo 1: 5′-AAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG GTCGCCGTATCATT-3′ Inverted dT (SEQ ID NO. 913, where the last T is labelled with an inverted dT) Blocking Oligo 2: 5′-CCTCAGCAAGAGCACACGTCTGAACTCCAGTCAC-NN-NNNNN-ATCTCGTATGCCGTCTTCTGCTTG-3′ Inverted dT (SEQ ID NO. 914, where N can be any of A, T, C, and G, and the last G is labelled with an inverted dT) The hybridization mixture is heated for 5 minutes at 95° C., then held at 67.5° C. 25 ul pre-warmed (67.5° C.) 2.8× hybridization buffer (14×SSPE, 14×Denhardt's, 14 mM EDTA, 0.28% SDS) was added. ..

    Article Title: Deconwolf enables high-performance deconvolution of widefield fluorescence microscopy images.
    Article Snippet: We stored the cells in 2× SSC supplemented with 0.05% NaN3 (Merck, cat. no. S2002) at +4 °C for up to 1 month until hybridization. .. Prior to hybridization, we incubated the cells in 50% formamide, 2× SSC and 50 mM phosphate buffer overnight at room temperature, followed by 1 h of incubation with a pre-hybridization buffer containing 50% formamide, 2× SSC, 5× Denhardt’s solution (Thermo Fisher Scientific, cat. no. 750018), 50 mM sodium phosphate buffer (home made from sodium dihydrogen phosphate (Merck, cat. no. 7558-80-7) and disodium hydrogen phosphate (Merck, cat. no. 7558-79-4)), 1 mM EDTA (Thermo Fisher Scientific, cat. no. AM9260G), 100 mg ml−1 salmon sperm DNA (Thermo Fisher Scientific, cat. no. 15632011) and 100 mg ml−1 human Cot-1 DNA (Thermo Fisher Scientific, cat. no. 15279011), pH 7.5–8 at 37 °C in a humidity chamber. ..

    Article Title: A Mammalian Conserved Circular RNA CircLARP1B Regulates Hepatocellular Carcinoma Metastasis and Lipid Metabolism.
    Article Snippet: .. After washing with PBST buffer twice and 2× SSC once in dark, samples were incubated with RNA probes mixed with 20 ng μL−1 human Cot-1 DNA (Invitrogen, 15279011), 500 ng μL−1 yeast total RNA (Invitrogen, AM7118) and 0.1 U μL−1 RNase inhibitor in 2× hybridization buffer (4× SSC, 40% dextran sulfate) at 37 °C for 15–17 Adv. ..

    Incubation:

    Article Title: Deconwolf enables high-performance deconvolution of widefield fluorescence microscopy images
    Article Snippet: We stored the cells in 2× SSC supplemented with 0.05% NaN 3 (Merck, cat. no. S2002) at +4 °C for up to 1 month until hybridization. .. Prior to hybridization, we incubated the cells in 50% formamide, 2× SSC and 50 mM phosphate buffer overnight at room temperature, followed by 1 h of incubation with a pre-hybridization buffer containing 50% formamide, 2× SSC, 5× Denhardt’s solution (Thermo Fisher Scientific, cat. no. 750018), 50 mM sodium phosphate buffer (home made from sodium dihydrogen phosphate (Merck, cat. no. 7558-80-7) and disodium hydrogen phosphate (Merck, cat. no. 7558-79-4)), 1 mM EDTA (Thermo Fisher Scientific, cat. no. AM9260G), 100 mg ml −1 salmon sperm DNA (Thermo Fisher Scientific, cat. no. 15632011) and 100 mg ml −1 human Cot-1 DNA (Thermo Fisher Scientific, cat. no. 15279011), pH 7.5–8 at 37 °C in a humidity chamber. ..

    Article Title: Methods for barcoding macromolecules in individual cells
    Article Snippet: Following washing, cells are incubated in pre-hybridization buffer, consisting of 2× saline-sodium citrate buffer (SSC; Ambion, AM9763), 50% formamide (Ambion, AM9342) and 0.1% of Tween-20 (Sigma-Aldrich, P4916) for 30 minutes. .. Next, the cells are incubated in hybridization buffer (2×SSC, 50% formamide, 10% dextran sulfate (Sigma-Aldrich, D8906) containing a mixture of CBPs at ̃20 nM total concentration with or without 10 μg Human Cot-1 DNA (ThermoFisher, 15279011). ..

    Article Title: Deconwolf enables high-performance deconvolution of widefield fluorescence microscopy images.
    Article Snippet: We stored the cells in 2× SSC supplemented with 0.05% NaN3 (Merck, cat. no. S2002) at +4 °C for up to 1 month until hybridization. .. Prior to hybridization, we incubated the cells in 50% formamide, 2× SSC and 50 mM phosphate buffer overnight at room temperature, followed by 1 h of incubation with a pre-hybridization buffer containing 50% formamide, 2× SSC, 5× Denhardt’s solution (Thermo Fisher Scientific, cat. no. 750018), 50 mM sodium phosphate buffer (home made from sodium dihydrogen phosphate (Merck, cat. no. 7558-80-7) and disodium hydrogen phosphate (Merck, cat. no. 7558-79-4)), 1 mM EDTA (Thermo Fisher Scientific, cat. no. AM9260G), 100 mg ml−1 salmon sperm DNA (Thermo Fisher Scientific, cat. no. 15632011) and 100 mg ml−1 human Cot-1 DNA (Thermo Fisher Scientific, cat. no. 15279011), pH 7.5–8 at 37 °C in a humidity chamber. ..

    Article Title: A Mammalian Conserved Circular RNA CircLARP1B Regulates Hepatocellular Carcinoma Metastasis and Lipid Metabolism.
    Article Snippet: .. After washing with PBST buffer twice and 2× SSC once in dark, samples were incubated with RNA probes mixed with 20 ng μL−1 human Cot-1 DNA (Invitrogen, 15279011), 500 ng μL−1 yeast total RNA (Invitrogen, AM7118) and 0.1 U μL−1 RNase inhibitor in 2× hybridization buffer (4× SSC, 40% dextran sulfate) at 37 °C for 15–17 Adv. ..

    other:

    Article Title: A Mammalian Conserved Circular RNA CircLARP1B Regulates Hepatocellular Carcinoma Metastasis and Lipid Metabolism
    Article Snippet: With three 5 min washes in PBST buffer (PBS with 0.4% Tween‐20), samples were incubated with Donkey anti‐Rabbit IgG Secondary Antibody Alexa Fluor 546 (Invitrogen, A10040) (1:200 diluted in 1% BSA) for 1 h at room temperature in dark.

    Concentration Assay:

    Article Title: Methods for barcoding macromolecules in individual cells
    Article Snippet: Following washing, cells are incubated in pre-hybridization buffer, consisting of 2× saline-sodium citrate buffer (SSC; Ambion, AM9763), 50% formamide (Ambion, AM9342) and 0.1% of Tween-20 (Sigma-Aldrich, P4916) for 30 minutes. .. Next, the cells are incubated in hybridization buffer (2×SSC, 50% formamide, 10% dextran sulfate (Sigma-Aldrich, D8906) containing a mixture of CBPs at ̃20 nM total concentration with or without 10 μg Human Cot-1 DNA (ThermoFisher, 15279011). ..

    Blocking Assay:

    Article Title: Nucleic acid capture method
    Article Snippet: .. A hybridization mixture was prepared containing 500 ng DNA library, 2 ug of RNA probes (1 ug from “+” clone transcripts and 1 ug from “−” clone transcripts, targeting Hg19 human exomes including −50 bp and +50 bp flanking intronic sequences, as well as 5′ and 3′ UTRs as described before), 7 ul Human Cot-1 DNA (ThermoFisher Scientific), 3 ul Herring Sperm DNA Solution (ThermoFisher Scientific), 10 μl blocking Oligos (1 nmol/ul each) with following sequences: Blocking Oligo 1: 5′-AAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG GTCGCCGTATCATT-3′ Inverted dT (SEQ ID NO. 913, where the last T is labelled with an inverted dT) Blocking Oligo 2: 5′-CCTCAGCAAGAGCACACGTCTGAACTCCAGTCAC-NN-NNNNN-ATCTCGTATGCCGTCTTCTGCTTG-3′ Inverted dT (SEQ ID NO. 914, where N can be any of A, T, C, and G, and the last G is labelled with an inverted dT) The hybridization mixture is heated for 5 minutes at 95° C., then held at 67.5° C. 25 ul pre-warmed (67.5° C.) 2.8× hybridization buffer (14×SSPE, 14×Denhardt's, 14 mM EDTA, 0.28% SDS) was added. ..

    Article Title: Nucleic acid capture method
    Article Snippet: .. Shear with below setting for a target size range of 100-150 nt: Setting value Intensity H On:Off 30:30 Cycles 35 Hybridize the probes with the DNA library Mix the following components as DNA library+block mix at room temperature: 4.3 μl of DNA library (150 ng/ul) 3 μl of Human Cot-1 DNA (Life Technologies 15279-101) 3 μl of Salmon sperm (Life Technologies 15632-011) 0.7 μl Customized blocking oligos mix (1000 μM), sequence shown below: Blocking Oligo 1 (as set forth in SEQ ID NO. 913) Blocking Oligo 2 (as set forth in SEQ ID NO. 914) Mix well by pipetting. ..

    Shear:

    Article Title: Nucleic acid capture method
    Article Snippet: .. Shear with below setting for a target size range of 100-150 nt: Setting value Intensity H On:Off 30:30 Cycles 35 Hybridize the probes with the DNA library Mix the following components as DNA library+block mix at room temperature: 4.3 μl of DNA library (150 ng/ul) 3 μl of Human Cot-1 DNA (Life Technologies 15279-101) 3 μl of Salmon sperm (Life Technologies 15632-011) 0.7 μl Customized blocking oligos mix (1000 μM), sequence shown below: Blocking Oligo 1 (as set forth in SEQ ID NO. 913) Blocking Oligo 2 (as set forth in SEQ ID NO. 914) Mix well by pipetting. ..

    Sequencing:

    Article Title: Nucleic acid capture method
    Article Snippet: .. Shear with below setting for a target size range of 100-150 nt: Setting value Intensity H On:Off 30:30 Cycles 35 Hybridize the probes with the DNA library Mix the following components as DNA library+block mix at room temperature: 4.3 μl of DNA library (150 ng/ul) 3 μl of Human Cot-1 DNA (Life Technologies 15279-101) 3 μl of Salmon sperm (Life Technologies 15632-011) 0.7 μl Customized blocking oligos mix (1000 μM), sequence shown below: Blocking Oligo 1 (as set forth in SEQ ID NO. 913) Blocking Oligo 2 (as set forth in SEQ ID NO. 914) Mix well by pipetting. ..



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    Image Search Results


    Reagents and tools table

    Journal: EMBO Molecular Medicine

    Article Title: Aberrant fragmentomic features of circulating cell-free mitochondrial DNA as novel biomarkers for multi-cancer detection

    doi: 10.1038/s44321-024-00163-6

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: Human Cot-1 DNA TM , Thermo Fisher , Cat # 15279101.

    Techniques: Sequencing, Software